FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005071985

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005071985
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8961844
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG113480.12662572568770447No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACCGG9950.045.0781061
TGACGCG3300.038.6841551
TGACGGC6450.038.5139581
GACCGCG4500.038.3341562
GACCGGG9600.037.7351842
TATGCCG7000.037.45731745
GACGGCG2850.036.316572
TGACGGG10350.035.0021171
TGACCCG9150.034.690621
TAACCGG3950.032.3184131
GACGCGG3650.032.1376762
ACCGCGG3550.032.071293
TGGCGGC11000.031.0518761
CTCGTAT8100.030.24054541
TGGCGGG16350.029.9651151
TGACCAG48850.029.5933971
TAGCCCG5150.029.4775121
GACCGGT3300.029.2733542
GACCGGC5150.028.8064442
GACCCGG9050.028.5917722
Produced by FastQC (version 0.11.5)