FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005071986

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005071986
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8961844
Sequences flagged as poor quality0
Sequence length75
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGG3350.020.5972669
TAGATCG6900.018.50022169
GTCGCCG5500.016.30928468
TCGCCGT3201.7007551E-915.09392968
CTACTCG27000.014.82239837
TAGCCGG31350.014.6380082
TCTCGGG8550.014.52648869
GCGCCTG25550.014.17769721
GGCGCCT17500.013.99676220
TTAGCCG30050.013.4349471
TACTCGG27600.013.37515938
CCTAGTC20050.013.24886916
CGCCGTA3406.114715E-813.19133368
GCTACTC74700.013.11662236
GTAGTCC69700.012.91851527
TCGTGTT28350.012.77792969
ACTCGGG27900.012.736567539
CAGCTAC126550.012.45879134
AGCTACT130100.012.41060135
CTCGGGA27300.012.13174240
Produced by FastQC (version 0.11.5)