FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005071988

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005071988
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6612811
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC24950.052.26767311
CCGATCT26100.050.09687412
TTCCGAT26500.049.34069410
CTTCCGA27850.046.8250779
CGTGTGC29850.046.0027161
TCTTCCG29050.045.365868
CGATCTA6650.035.79640213
CGACGCT1207.8216544E-1131.6268671
GTGCTCT44550.029.9694254
CGATCTG7700.028.67484713
GTGTGCT48200.028.3437162
TGTGCTC49000.027.669743
CGATCTT15050.027.27861813
GCTCTTC50100.026.9247046
TGCTCTT66200.020.220215
CTCTTCC76500.017.5428477
CGATCTC27550.017.15579613
GCGCGAG1450.005218144514.275949558
CGTTGGA23300.013.3269671
AACGGGA21300.012.3096568
Produced by FastQC (version 0.11.5)