FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005071989

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005071989
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5952064
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT25850.065.4064561
GACGCTC27450.061.341112
TCCGATC29750.057.74936710
ACGCTCT29350.057.4852753
TTCCGAT30750.055.9835249
CCGATCT30850.055.57793411
CTTCCGA31750.054.437588
CGCTCTT31550.054.3521274
TCTTCCG34100.050.8883557
CGATCTA7450.047.2329512
CGATCTG8350.034.29199612
GCTCTTC52400.033.317715
CGATCTT15250.032.80192612
CTCTTCC75750.023.3663356
CGATCTC26500.022.26134712
TATGCCG3700.018.64881949
CGTATGC3650.017.95861447
TGCCGTC4050.017.88905351
TCTCGTA3703.6379788E-1215.8508344
GCCGTCT4800.015.09388852
Produced by FastQC (version 0.11.5)