FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005071992

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005071992
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8017933
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC41850.052.5943611
CCGATCT42150.052.13817212
TTCCGAT42250.052.01476710
CTTCCGA43600.050.7207269
CGTGTGC45800.049.2663731
TCTTCCG46050.048.396828
CGATCTA10000.043.81475413
GTGCTCT65300.034.6056864
CGATCTT22600.034.34715313
GTGTGCT68300.033.4897732
CGATCTG10850.033.3869113
TGTGCTC69200.032.7052273
GCTCTTC72050.031.3634626
CGCCGTA1254.1600288E-927.60018759
TGCTCTT95950.023.6590655
CTCTTCC102400.022.4382117
TCGCCGT1751.4754733E-719.71429858
CGATCTC34200.019.67094213
GTCGCCG1952.1591404E-819.46155257
CGACGCT1801.9840991E-719.1675171
Produced by FastQC (version 0.11.5)