FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005071998

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005071998
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6721515
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA3800.053.5663159
TCCGATC26500.050.3827611
TCGCCGT4300.048.94233758
CCGATCT27750.048.3619112
TTCCGAT27600.048.25082410
GTCGCCG4600.047.25045857
CTTCCGA29100.045.882239
CGTGTGC30050.045.5808371
TCTTCCG31500.042.7147038
CGATCTA7150.040.53125813
GTACGTC5400.039.611232
GCCGTAT5550.039.1619660
GGTCGCC5900.038.0085656
TACGTCC5900.035.6695733
TGGTCGC5850.034.79469355
CCGTATC6200.031.71757761
CGATCTG8200.031.55471413
GTGGTCG6650.030.60886654
GTGTGCT47550.029.3851412
GTGTACG7350.029.10210630
Produced by FastQC (version 0.11.5)