FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005071999

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005071999
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8355321
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT35350.066.5669861
GACGCTC39050.060.3466572
TCCGATC41600.057.2222810
TTCCGAT42600.056.0406659
CTTCCGA43200.055.5018968
ACGCTCT42500.055.284573
CCGATCT42950.055.26302311
TCTTCCG45500.052.6204877
CGCTCTT45200.052.592834
CGATCTA9700.048.01410312
CGATCTT22800.034.0450912
GCTCTTC73650.032.8861275
CGATCTG11050.031.8452512
CGATCTC34350.023.3006512
CTCTTCC106200.023.0663876
CGCCGCT3052.333054E-713.5751731
ACCGCGC10200.011.83867931
CGTTGGA28500.011.74331951
AAACGGG30800.011.537017
CACCGCG11450.011.45021130
Produced by FastQC (version 0.11.5)