FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072003

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072003
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7678392
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT29350.066.538311
GACGCTC31900.060.5692672
TCCGATC33950.057.41332210
ACGCTCT34350.056.448973
CCGATCT35300.055.4127311
TTCCGAT35250.055.393839
CTTCCGA35550.055.1204538
CGCTCTT36800.053.3474584
TCTTCCG38500.050.896947
CGATCTA7750.044.95973212
CGATCTT18100.033.545812
GCTCTTC60650.032.824215
CGATCTG10750.032.0919112
CTCTTCC91950.021.7631976
CGATCTC30850.021.5827212
TATGCCG3703.6379788E-1215.85131849
CGCCGCT2907.536073E-915.4670961
CGTTGGA23300.014.3641281
ACGGGAT15700.013.843479
AACGGGA21650.013.5445658
Produced by FastQC (version 0.11.5)