FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072004

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072004
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7678392
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC27200.050.9871711
CCGATCT28700.048.44285612
CTTCCGA29300.047.33289
TTCCGAT29500.046.89495510
CGTGTGC32350.044.794141
TCTTCCG32800.042.7027638
CGATCTA7650.039.2344213
CGATCTT14000.030.55646113
GTGCTCT52800.027.3115374
GTGTGCT54050.027.2550662
TGTGCTC55800.026.2142983
CGCCGTA1901.8189894E-1225.42197459
GCTCTTC56850.024.880396
TCGCCGT1951.8189894E-1224.76964858
CGATCTG11200.021.87004913
GTCGCCG2402.910383E-1120.12520657
CGACGCT1056.024732E-419.7155171
TGCTCTT77100.018.6588755
CTCTTCC86350.016.3404777
CGTTGGA23450.016.3315091
Produced by FastQC (version 0.11.5)