FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072005

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072005
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6395818
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT29700.066.455431
GACGCTC32350.061.1163832
TCCGATC34850.058.3067110
ACGCTCT34150.057.7917253
CCGATCT35800.056.66309411
TTCCGAT36000.056.443699
CTTCCGA36850.055.235358
CGCTCTT36900.054.3262984
TCTTCCG37900.053.4320077
CGATCTA8250.045.9986112
CGATCTT16650.039.1609812
CGATCTG10650.037.252412
GCTCTTC59350.034.5323455
CTCTTCC82700.024.9491656
CGATCTC26800.023.55712512
TATGCCG3251.2369128E-1015.92247249
ACCGCGC6350.013.58343631
CGTTGGA21700.013.5160591
TCTCGTA3651.0837539E-813.23268444
CGCCGCT2355.2254276E-513.214951
Produced by FastQC (version 0.11.5)