FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072007

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072007
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6227246
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT24200.067.008961
GACGCTC26850.060.3949282
TCCGATC28850.056.91971210
ACGCTCT28550.056.675613
TTCCGAT29600.055.3609359
CCGATCT29950.054.9443611
CGCTCTT30700.053.7174644
CTTCCGA30800.053.428048
TCTTCCG32750.050.6677787
CGATCTA6600.045.99814612
CGATCTT14800.034.964812
CGATCTG8600.033.2951712
GCTCTTC51850.032.1384055
TATGCCG3050.024.88464249
CGCCGCT2900.023.7948171
CTCGTAT3550.023.3231945
CGATCTC25650.021.92309212
CTCTTCC77150.021.822776
TCTCGTA3800.019.97444
CGTATGC4400.018.03336147
Produced by FastQC (version 0.11.5)