FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072010

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072010
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9148961
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC33550.051.10547611
TTCCGAT35250.048.93415510
CCGATCT35100.048.84868212
CTTCCGA36400.047.293649
CGCCGTA2700.046.00137359
CGTGTGC39600.044.783351
TCTTCCG38850.044.399968
CGATCTA9000.039.48192213
TCGCCGT3400.035.51537758
GTCGCCG3550.034.98657657
CGATCTT19350.031.5570113
GTGCTCT62050.029.0231254
GTGTGCT63050.028.7274322
GCCGTAT4500.027.60097560
CGATCTG10650.027.5342313
TGTGCTC66800.027.0626473
GCTCTTC70400.025.2370246
GGTCGCC4850.024.89724256
TGGTCGC5150.024.11577455
GTGGTCG5900.020.46581654
Produced by FastQC (version 0.11.5)