FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072013

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072013
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7171794
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT33800.067.068461
ACGCTCT37750.060.2314073
GACGCTC37500.060.174592
TCCGATC38850.058.5198910
CCGATCT39450.057.45455611
TTCCGAT40200.056.5546729
CTTCCGA40850.055.4858748
CGCTCTT40950.055.0191464
TCTTCCG41350.054.731517
CGATCTA8300.046.96847512
CGATCTT20850.040.3728912
CGATCTG9050.038.1204112
GCTCTTC66900.034.399675
CTCTTCC95050.024.2116996
CGATCTC29250.024.17876212
CGTTGGA16100.012.2157091
ACGGGAT12650.011.1815629
AACGGGA15600.011.0574398
CGGGATT12300.010.377810510
AAACGGG18950.010.3770717
Produced by FastQC (version 0.11.5)