FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072015

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072015
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6941524
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT26300.065.20351
GACGCTC28300.060.2266732
ACGCTCT30300.056.2500953
TCCGATC31100.054.91052210
CCGATCT32200.053.3557411
TTCCGAT32350.052.7887889
CGCTCTT32400.052.7111244
CTTCCGA33100.051.69698
TCTTCCG34600.049.854547
CGATCTA7550.037.01173812
CGATCTT17800.034.30478312
CGATCTG8500.032.46926512
GCTCTTC60650.028.9557825
CTCTTCC85100.020.4743526
TCGACGC850.00411943120.2965091
CGATCTC32050.017.76058812
CGTTGGA21200.015.9499911
AAACGGG24250.013.9420017
ACGGGAT14450.013.8474869
AACGGGA20400.013.6982688
Produced by FastQC (version 0.11.5)