FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072016

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072016
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6941524
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC61550.060.64730511
CCGATCT61800.060.17867312
TTCCGAT62900.059.5650510
CTTCCGA64050.058.7648939
CGTGTGC64000.058.599831
TCTTCCG65500.057.5693328
CGATCTA13600.054.0322513
CGATCTG16700.046.48131613
GTGTGCT82950.045.2922442
CGATCTT25200.043.8088413
GTGCTCT85350.043.73574
GCTCTTC88500.043.0756036
TGTGCTC88550.042.116233
TGCTCTT109050.034.989835
CTCTTCC116000.033.1016437
CGATCTC36800.031.31201613
CGCCGTA1555.2750693E-1126.70983959
CGACGCT1208.525785E-825.8766461
TCGCGAG950.00783902918.1576127
TCGCCGT2401.232911E-817.2499858
Produced by FastQC (version 0.11.5)