FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072018

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072018
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4595541
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA1551.0044077E-620.03517769
TGGTCGC6250.016.56241469
TCGCCGT2152.2626631E-514.44396568
TTAGCCG13800.013.5024081
TAGCCGG14350.013.2227662
CGGGCGT5750.012.5997796
GTGGTCG7600.012.25836568
GGTCGCC4308.210918E-912.03663769
CTCGGTG15600.011.72286269
TCTCGGT16250.011.67862469
GCGTCGG5158.421921E-1011.39000969
GCCGTAT2150.005315610711.23419568
CCGTATC2550.001529603710.82510769
GTCGCCG2954.339748E-410.526957567
AGCCGGG20800.010.2834733
GTGGCGG11850.010.18980614
TCTCGGG3055.900403E-410.18181169
GTAGTCC30200.010.16715127
GGTGGCG16200.09.79612413
TCGGTGG12700.09.78095269
Produced by FastQC (version 0.11.5)