FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4723394
Sequences flagged as poor quality0
Sequence length75
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACT4600.047.2917481
ACGCCCT7650.041.5268551
AGCCCAT42750.040.5480731
ACGACCT4350.040.4840161
ACGCCTT8550.040.3865281
ACGACCA4950.039.7623751
CGGACCT6300.038.3672031
ACGCCTC8300.038.274751
CGGACTG6250.038.1216551
AGCGCCA5400.037.727751
ACGGCCA6800.037.577291
CGGTCCT8150.037.284451
CGCCCAT12850.037.083321
ACGCCTA5800.036.9118961
CGGCGAT1500.036.8325161
CGGTATC5350.036.7894861
ACGACTC3300.036.6232381
CGGTATG5100.036.5616841
CGGGGAT9200.036.407141
CGGCATA5900.036.286271
Produced by FastQC (version 0.11.5)