FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4152080
Sequences flagged as poor quality0
Sequence length75
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTATC4450.046.5665441
CGGACCG751.6370905E-1146.0491371
CGGGCCA8600.043.371861
CGGTATT7100.043.292681
CGGTGTA4900.042.9948581
CGGTACT5000.042.82571
ACGCCTT7100.041.8333741
ACGACTA2000.041.4442251
CGACCTA2750.041.3993072
CGGACCT6900.041.0437971
CGGGCCT10950.041.0026551
ACGACCC3300.040.816281
ACGCCTC6300.040.0188941
AGCGCCA5700.039.990041
ACGACCT4700.039.680641
CGCGCTA702.1709639E-839.4706881
CGGTATA6150.039.3102381
AGCCCAT37000.038.9239651
CCGCCTA5300.038.4466861
ACGACCA4150.038.2818151
Produced by FastQC (version 0.11.5)