FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072031

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072031
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4939593
Sequences flagged as poor quality0
Sequence length75
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTA800.051.7968561
CGGTACA5700.043.6184041
ACGCCTT7550.042.5351681
AGCCCAT44100.042.5180551
ACGACCC3600.042.2048451
ACGCCTC8150.041.098531
CGGGACG1200.040.286441
CGGGGTG9800.040.168991
CGGTCTG7850.040.0298421
ACGACCT5350.040.0175061
CGGTACT6050.039.9534951
ACGCCCT6750.039.9027631
CGGTATG6750.039.9027631
CGCCCAT14200.039.8811451
ACGACCA5550.039.81981
CGGTCCT7700.039.464271
CGGGCCA11450.039.205771
CGGCCCA14400.039.087441
CGGTCTT9550.038.689451
CGGGCTT8400.038.6420971
Produced by FastQC (version 0.11.5)