FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072035

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072035
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3911226
Sequences flagged as poor quality0
Sequence length75
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCCT5950.043.5224081
CGGTATA5550.042.9264261
ACGCCTT7450.042.638331
CGGTACT3650.041.6225241
ACGACTG3900.041.6103971
GCGTACT1850.041.060061
ACGACCA5400.040.921811
AGCCCAT37900.040.540531
CGACCTA2050.040.389692
ACGCCTC5950.039.4603161
CGGACCA4400.039.236111
CGGCGTA459.972693E-538.3641971
AGCGGAC2250.038.3641971
ACGGCTA2700.038.3641971
CGCCCCA11950.038.1394651
ACGGCCT5550.037.9494481
CGGGCTA4650.037.8691751
CGGTCCT6400.037.7647551
ACGACCT5350.037.4319841
AGCCCTA22300.037.1599351
Produced by FastQC (version 0.11.5)