FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072040

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072040
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12143702
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA286850.23621297689946608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAAAAA491350.016.4883421
TAAAAAA425000.013.4893591
CAAAAAA381300.09.9026521
TCGTGTA26100.08.72537769
CGTGTAG25150.08.09458869
GTCGTGT29500.08.07063768
CGTCGTG30450.08.04531167
GCGTCGT30150.07.89648166
TACGCTA3950.0062059717.85993345
CGTTATA6151.381226E-57.85377144
TTCGAAC11901.8189894E-127.82781141
GCGTCGG11554.1927706E-96.871105769
AGCGTCG36500.06.80644669
ATTTCGA15555.456968E-126.65631739
TCGAACA15352.3646862E-116.518008742
AATTTCG16301.6370905E-116.350071438
ACGAAAC14251.2132659E-96.29456748
GATCGGA92100.06.1067369
AGAGCGT52450.06.05233768
GAAAGAA245000.05.969941
Produced by FastQC (version 0.11.5)