FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072044

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072044
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12682730
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA324390.255773007861872No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAAAAA527350.017.657641
TAAAAAA456700.015.7859341
CAAAAAA400400.011.2524111
ACGTTAC10203.6379788E-118.11695722
CGTTACC12600.07.93980123
ACGAAAC13751.8189894E-127.27617548
TTCGAAC15850.07.18289341
GAACGTT14303.6379788E-126.995908720
GAGCGTC65750.06.927039669
AGAGCGT67800.06.921157468
CGCTACG5050.0071408676.83137259
CGAAACA18600.06.67693149
TCGTGTA34150.06.466343468
CGTGTAG35250.06.362440669
CGTTAAA13602.7011993E-96.35618161
GTCGTGT37750.06.12388969
CGTCGTG41500.06.06937968
GCGTCGT41050.06.051716367
AACGTTA13702.2060703E-86.043281621
TCGAACA16651.891749E-106.00906742
Produced by FastQC (version 0.11.5)