FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072051

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072051
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13366121
Sequences flagged as poor quality0
Sequence length75
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATA9300.035.2668841
CGGTTTA16950.033.4041631
CGGGTAT17600.031.9743251
GCGATTA12250.031.565191
CGGTTAT20700.031.3555151
GCGATAT13050.029.630161
GGGTTAT52550.029.4985081
CGGATTA14750.029.2579881
CGGTAAT15950.029.2212981
CGGATAT20750.028.9506021
CGGTTAC19700.028.7411461
GGGTATA40200.028.6844731
GCGATTT23600.028.5265391
CGGACTA10100.028.3715571
CGGGTTT29850.028.2209681
CGGTATA16100.028.0913051
CGGTTGT26350.027.907791
CGGTCTA12000.027.9072441
GCGTTCT37050.027.8618181
CGGTTAG11400.027.8618141
Produced by FastQC (version 0.11.5)