FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072052

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072052
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13366121
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA391720.2930693205605426No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAAAAA570550.017.0722541
TAAAAAA506150.014.1344521
CAAAAAA437400.010.7749391
CGTATCG2700.00246644610.22129121
TCGTGTA33000.07.214490469
CGTCGTG39200.07.0416568
ACGTTAC11051.26110535E-86.868126422
GTCGTGT37700.06.864207769
GCGTCGT40250.06.857852567
CGTGTAG33500.06.69482369
AACGTTA12908.749339E-106.68543721
AGCGTCG50400.06.572206569
GCGTCGG15059.822543E-116.41936569
GGATACG10055.684735E-66.178818759
CGTTAAA11601.4421585E-65.96169471
GATACGT8701.9725766E-45.947956660
GAACGTT14551.2649252E-85.92729520
ACTCGTG14104.0472514E-85.87237633
GAAAGAA270550.05.84071971
CGTCTTA7700.00180167915.82412246
Produced by FastQC (version 0.11.5)