FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072055

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072055
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13699648
Sequences flagged as poor quality0
Sequence length75
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTTA17250.033.4263761
GCGTTAT14600.033.1083151
CGGTCTA12600.032.6090621
CGGGTAT18950.030.9743061
CGGTTAT20600.030.504651
GCGTATT16900.030.2368761
GCGTATA11200.029.9030551
GCGATAT16050.029.6869751
GGGGTAT44000.029.5835691
CGGTTCT42450.029.0370451
GCGATTT26950.028.9541991
CGGTTAG10550.028.7999741
GGGGGGG73750.027.949511
GGGTATT61650.027.7226031
GGGTTAC47400.027.6072251
CGGTTAC19650.027.235231
CGGATAG14900.027.1119961
GGGTTAT53000.026.6446061
CGGTATT24450.026.2661231
CGGATTA14200.026.2601551
Produced by FastQC (version 0.11.5)