FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072059

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072059
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12237801
Sequences flagged as poor quality0
Sequence length75
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATAT12650.034.1174931
CGGTTTA16100.033.2401851
CGGACTA8600.032.519521
GCGTATA7350.032.413011
GCGATTT22650.030.1824571
CGGTTAT17750.029.3721791
CGGGTAT16050.029.2564411
CGGATTA12800.028.8623311
GCGATTA11850.028.8452591
GGGGTAT38700.028.6385921
GGGTTAT45900.028.5843641
CGGTTAG10900.028.5084511
GCGCATT16850.028.482131
CGGTATA13850.028.4192541
CGGGTTA11750.028.2092111
CGATATA8950.027.7538172
GCGTATT14350.027.6696431
CGGTTTG27100.027.3921931
CGGTCTA11350.027.378161
GGGTATA35600.027.3499581
Produced by FastQC (version 0.11.5)