FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072064

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072064
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5485009
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA261860.4774103378864101No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG17000.022.73320869
CGCCGTA3150.018.62215669
GTCGTGG10850.017.80942569
GCCGTAT3951.5643309E-1013.97701669
TCGCCGT4253.45608E-1113.80230468
GGCTCGA2751.0796102E-613.79978831
CGGTCAT2304.2658976E-513.5034871
CGAATCT3551.074859E-712.6336135
GTCGCCG5105.4569682E-1112.17850467
TCGGTGG18500.011.37757569
TGGTCGC9050.011.05709669
TCTCGGG5108.762072E-910.82533769
TCGTGTG5754.5292836E-1010.80180469
GGTGGCG16950.010.38037213
GCTCGAA4057.1318736E-610.22206632
GTAGTCC32150.010.08693827
AGAGCGG27200.09.89503669
GTGGTCG9900.09.75920668
CTACTCG12750.09.74102737
CGGTGGT16050.09.67451269
Produced by FastQC (version 0.11.5)