FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072068

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072068
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5174146
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA199530.3856288554671631No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTGG13100.023.9703469
GCGTCGG24950.022.12857869
CGTCGTC1451.2641729E-519.03802567
CGCCGTA1804.194484E-617.25320867
CGTCGCG2101.8110777E-514.78860768
GGTCGCC5450.014.56248269
TCGTGTG7000.014.29565469
CGGACTA1450.005210714414.2789321
TCGTCTA2153.6518252E-412.83972269
TCGCCGT2457.719183E-512.6759568
GTCGTCT1950.002560898212.38703968
CCGTCGG2255.357334E-412.26906769
CGTCGAG4256.897608E-912.17885468
GCCGTAT2601.3445683E-411.94464568
TCTCGGG3308.2952465E-611.50225169
TGGTCGC6600.011.50225168
GCGTCGC2701.9105365E-411.50213967
TTCGTAT4501.6138074E-811.49969410
AGAGCGG38150.011.39672669
GTAGGGG26750.011.35175369
Produced by FastQC (version 0.11.5)