FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072069

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072069
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4364928
Sequences flagged as poor quality0
Sequence length75
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCTT11100.052.8777431
CGGTACG800.051.7890851
GCGCATT7900.050.6964871
GCGTACC2650.049.5090641
GCGTACT3650.049.187811
GCGTCTA5200.047.805311
CGGTATG5650.047.053211
GCGGGTA5000.046.9554371
CCGACTA3750.046.9554371
CGGTATA8850.046.8149951
GCGTCCT8600.046.570031
CGGTAAT5650.046.442131
GCGTATC3950.046.32611
CCGTATA4850.046.2720341
GCGGGTT5100.046.0347441
GCGCCCT10250.045.810181
GCGGCTA4200.045.2126961
CCGTACT3600.045.0756841
GCGCGTA1150.045.033991
CGGTCCG1000.044.8838731
Produced by FastQC (version 0.11.5)