FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072070

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072070
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4364928
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA143420.3285735755549691No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG14250.025.66993169
TCGCCGT2750.020.07802469
GTCGCCG3700.017.7208968
GTCGTGG8550.016.54822569
GCGAATC1350.003245720215.33281159
GTCGTGC1550.00810113413.35836267
CGCCGTA1600.00999066212.94091467
TGGTCGC7250.012.37567869
GGTCGCC5451.4551915E-1112.03069667
CGGGCGT5753.6379788E-1211.9995926
CGATATC2050.00372566711.7821121
TCGTGCA2357.715964E-411.7477868
GGTGGCG14350.011.05885613
GTGGTCG8750.010.64852368
TAGCCGG12050.010.5932
CGGTGGT13750.010.54096268
GGCGCCT7600.010.44055420
TCTCGGG3351.21912315E-410.30122569
TCGGTGG14100.010.27930769
ATGGCGT4401.7265356E-610.19283662
Produced by FastQC (version 0.11.5)