FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072072

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072072
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5450237
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA145660.2672544331558426No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA2150.030.49258469
CGTCGTA700.00133341324.64626367
TCGCCGT2900.022.60657168
GCGTCGG16800.021.36009269
GTCGCCG4200.017.25238267
GTCGTGG10200.016.57581969
GGTCGCC6050.013.68784166
TCGTGTG4902.7284841E-1112.675220569
ACGCGAG2000.003101189912.07467659
CGTGGCG2000.003101189912.07467610
GGCGCCT9000.011.88334120
TAGCCGG13000.011.6766072
TGGTCGC7600.011.35025269
GGTGGCG16150.011.32167513
TCGGTGG17100.011.29980669
CTCGGTG20750.011.14129869
CTAGCCG2200.006304486710.9796961
TACGTAG2200.006314820610.97727917
GCCGTAT2250.007454926610.73481768
TTAGCCG13250.010.6778291
Produced by FastQC (version 0.11.5)