FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072073

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072073
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4887053
Sequences flagged as poor quality0
Sequence length75
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGTA1350.056.2772671
GCGTCTA5000.050.4193151
GCGTCTC14500.049.538111
CGGCGTA1750.049.333961
GCGCCTT15100.048.7132071
GCGCCTA8100.047.3240621
CGGGGTA5700.047.2567441
GCGTCCC10800.046.684551
CGGTATT11700.046.6353571
CGCGTAA751.6370905E-1145.9993362
GCGTCTT11950.045.9487041
GCGTACA4750.045.8026921
GGCGGTA3250.045.690841
CGGTACT5600.045.6339231
CGGACCA6000.045.4694671
GCGCCTC16700.045.2868081
CGGTCCA7550.045.28271
GCGCCCT12550.045.1278041
CGGTCTT13650.044.5270651
CGGTCCT10550.044.517471
Produced by FastQC (version 0.11.5)