FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072075

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072075
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4937821
Sequences flagged as poor quality0
Sequence length75
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCCT10750.050.753421
CGGTATG7250.050.4875831
GCGCCTT15050.050.4780921
GCGCCCT11950.049.702391
CGGTATA9150.049.438691
CGACCTA2100.049.2849042
GCGACTC5750.049.2450681
GCGTCCC8250.048.972091
GCGTCCA7800.048.6984141
GCGTCTA4700.047.756471
GCGCCTA6600.047.6117551
GCGACTG5200.047.148921
CGGTCCA7550.047.1093371
CGGTCTT12600.046.8643151
CGGTGTC8700.046.8359641
CGGTCCT10600.046.5850871
GCGTACC3600.046.0421371
GCGTATC3750.046.0421331
GCGCCTC14700.045.8072281
GCGACCA6650.045.6959571
Produced by FastQC (version 0.11.5)