FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072082

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072082
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3924401
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA2100.029.57456469
TCGCCGT2650.022.1344268
GCGTCGG6250.019.32204869
GTCGTGG5400.018.52974369
GTCGCCG3400.017.2516167
GCCGTAT2051.4430829E-515.14794668
TCTCGGG2302.5745067E-615.00159169
CCGTATC2101.812392E-514.78728269
CGTGGCG2001.9835225E-413.79988110
TGGTCGC6350.013.58411669
GGTCGCC5156.548362E-1112.05922366
TACTCGG8650.011.96536638
GTGGTCG8000.011.64498569
TTCGTAT3003.982822E-511.499910
TCGGTGG13700.011.33331869
CTACTCG8700.011.10335237
TAGCCGG10400.010.9470212
CTTCGTA3501.5969352E-510.8427639
AGAGCGG8350.010.74365469
GGCGCCT6654.9112714E-1110.3758520
Produced by FastQC (version 0.11.5)