FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072083

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072083
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4909180
Sequences flagged as poor quality0
Sequence length75
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGTA200.00736081751.7493632
GCGTCCC8900.051.2112051
CGGACCG750.050.642191
GCGTCTC11250.049.7214241
GGGTCCT36950.048.9663731
CGGTCCA5800.048.8165321
CGGTATA5750.048.6405221
GCGTCCA7250.048.5784031
CGGTACA6400.048.5560761
GGGTCCC30600.048.5208171
GGCGAAT2250.047.5729681
GCGCCTT12350.047.5294761
GCGGCTA4600.047.2893941
GGCCCTC67500.047.1637341
GGGTCTC42250.046.419741
GCGCCTC13700.046.37441
GGCTCCC54950.046.3734861
GGGCCCT31150.046.0014081
GGCCCAT44550.045.960851
GCGCCTA5950.045.8449131
Produced by FastQC (version 0.11.5)