FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072084

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072084
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4909180
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAT200.00736115651.7487621
GGCGCCT12050.030.34782220
GCGCCTG16300.027.30302821
CGGGCGT8950.026.9825946
TAGCCGG20950.026.841842
TCTCGGG2600.026.54377769
TTAGCCG19450.026.258521
CTACTCG15600.025.65427837
TACTCGG14550.025.6086838
CGGACGT700.001334653924.6422676
GTCGTGG35250.022.61303769
GTAGTCC50650.022.54559327
ACTCGGG14950.022.38476639
TCGTCTA1403.7736572E-722.18301269
CGTCGTC1403.7747668E-722.18233367
TGTAGTC55200.021.99969726
CCTGTAG56300.021.6309224
GCTACTC39650.021.31813836
TGGGCGT12600.020.8090276
TCTCGGT13950.020.77835369
Produced by FastQC (version 0.11.5)