FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072085

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072085
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4905177
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACTA3150.049.3199041
GCGCCTT11450.046.1324161
GCGCATA5500.044.5672571
CGGTACA6600.043.9395451
GCGCCTA7900.043.701181
GGCCCAT51550.043.4646571
CGGTCTT9400.043.338551
GCGACAT4950.043.2420961
GCGTACT4800.043.154911
GCGTACA4650.043.0621071
GCGTCCT8500.043.053371
CGGTATG5500.042.6841351
GCGGCTA4650.042.3196561
CGGTCTG8850.042.13091
CGGTATA7300.042.090821
GCGCCTC11350.041.9762341
GGCCCTT51750.041.8957061
GCGCACT4550.041.7322241
CGGTACT5250.041.428721
GGCGGTA4100.041.2603071
Produced by FastQC (version 0.11.5)