FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072086

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072086
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4905177
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA61030.12441956732652053No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA2901.8189894E-1219.03627869
GCGTCGG9950.018.72538269
GTCGCCG5750.016.80158469
TCTCGGG3900.015.92457769
TCGCCGT3808.367351E-1114.52768568
CTACTCG11500.014.39988937
TACTCGG11300.013.43352538
GGTCGCC7450.013.43079769
TAGCCGG13000.013.0037462
TTAGCCG12550.012.37130451
TGGTCGC9250.012.30926869
GCCGTAT2902.8138484E-511.89767469
CTTCGTA6100.011.8769589
CGGGCGT6500.011.6768336
GTCGTGG6600.011.50108369
GGCGCCT9300.011.49991120
ACTCGGG13550.011.45747739
CTCGGGA12350.011.45335440
TCGGTGG18650.011.28524668
GTAGTCC30050.011.25116227
Produced by FastQC (version 0.11.5)