FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072087

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072087
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4724173
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACT5300.048.8533671
CGGTACG950.047.2420651
GCGACCA5700.046.6363951
CGGTATA6500.046.207771
GCGCCTA7450.045.8762631
CGGACCA5800.045.23711
CGGTACA5350.045.170341
GGCCCAT50100.044.5147441
GGCCCCC31950.044.4099271
CGGTCCT6150.043.7853281
GCGTCCA7100.043.761611
GCGACTT5400.043.4734651
GCGTCTT8200.043.3643151
GCGTACT4550.043.2486531
GCGCCCT8650.043.103921
CGGTATG6000.042.5784261
GCGACAC3700.041.987491
GGCCCTA31750.041.753861
GGCCCTT45700.041.6240651
CGGGCCA9000.041.4276541
Produced by FastQC (version 0.11.5)