FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072088

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072088
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4724173
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA57420.12154508312883547No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG11100.022.69230369
GTCGTGG6350.018.47497469
CGCCGTA1400.004129600214.78774369
TTAGCCG10600.013.6719761
TCTCGGG2851.6108952E-613.31761669
GTCGCCG2609.159137E-613.2710568
TAGCCGG11100.013.053912
GTGGTCG6150.012.90420969
AGAGCGG14800.012.35642369
TCGCCGT2100.004460507511.50157768
TCGGTGG10600.011.39307268
CGGGCGT5209.913492E-1011.2787226
GGATCGT2853.1600357E-410.89461763
GGTGGCG13050.010.57459513
TCTCGGT13600.010.40216269
GATCGTT3005.0746725E-410.34988664
CTCGGTG13700.010.32623469
GGCGCCT6706.184564E-109.78347420
CGGTGGT10600.09.76549168
ATCGTGC3956.0498576E-59.6074956
Produced by FastQC (version 0.11.5)