FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072091

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072091
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6772399
Sequences flagged as poor quality0
Sequence length75
%GC36

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGTA850.052.8214721
CGGCGAG1200.051.8056761
CGGTACA7450.048.6764761
GCGTACT4550.048.5796851
GCGTATC4950.048.142651
GCGTACA6600.047.6193581
CGGTCCA6300.047.1459051
GCGGTAC2200.047.096071
GCGCATA4900.046.5193861
GCGTATA6800.046.218791
CGGTGTA7850.046.1961441
GCGACTA3450.046.049491
GCGCGTC1650.046.049491
GCGGATA4450.045.7907871
GCGTCCA9450.045.3185421
CGGTATG7250.045.2555351
GCGCATT9850.045.2313541
GCGGCCA7650.045.1465641
CGGTATA9300.044.935391
GCGCCTT10500.044.733791
Produced by FastQC (version 0.11.5)