FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072096

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072096
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29206896
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1098910.3762501842030731No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN524230.17948843314263863No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG107850.020.4979112
TTAGCCG104600.020.2431741
CGGGCGT48200.020.052496
CGCCGTA40900.019.73152769
GCCGTAT38000.018.15158769
CCGTATC30650.018.11605369
GGCGCCT65900.016.6480820
AGCCGGG143900.016.3469283
TCGCCGT54700.015.38362868
GGTGGCG116400.015.26615413
CTACTCG87550.015.24853237
GTAGTCC210650.014.90188727
GCGCCTG88500.014.81349621
TACTCGG89700.014.57478338
GAACCGC18950.014.38269720
GCGTGGT153450.014.2596019
CCTAGTC69500.014.0992116
GTCGCCG73600.014.01075669
GTGGCGG87450.013.96733714
CGGTCTC23500.013.81687451
Produced by FastQC (version 0.11.5)