FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072098

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072098
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25078794
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA895960.3572580084991328No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN677000.26994918495682046No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACCGC41100.013.08332320
TCCGATC22800.012.24757230
TGAACCG36900.011.58320919
TCGGCAT44000.011.0954316
CGGTCTC47750.011.0037631
CGGCATT43950.010.867317
AACCGCT35200.010.5774721
ACCGCTC38900.010.54673622
CGAACCG9750.09.54536919
GTCTCGG54900.09.0212673
GGTCTCG52450.08.878992
CCGATCT30350.08.86039631
TCGTGTA107400.08.34675569
CTCGGCA67650.08.0887395
GTCGTGT121800.07.869655668
CGCTCTT48000.07.613639424
TCTCGGC66850.07.51233244
TTCCGAT34600.07.47207829
CGTCGTG137300.07.181978769
ACCCCGG76450.07.00850251
Produced by FastQC (version 0.11.5)