FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072102

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072102
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5361108
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA68220.12724981477709457No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG14800.028.90882569
GTCGTGG9450.020.44686369
TCTCGGG3050.019.23178169
CGCCGTA2603.302739E-815.9249668
GGTCGCC5700.013.92269969
CGGATAG1750.001134700613.8017621
AGAGCGG20600.013.23214769
TCGCCGT3154.9447845E-612.04893267
GCCGTAT3308.302546E-611.5013669
TAGCCGG12300.011.4998592
TGGTCGC6850.011.08160368
TCTCGGT15600.010.83782169
TTAGCCG12500.010.7653741
CCGTATC3551.8659863E-510.69140569
GTCGCCG4159.519201E-69.97708369
GTGGTCG8450.09.79997669
ATCTCGG23500.09.39685668
TCGGTGG13050.09.25396868
CGTCGGG8404.7293724E-119.03678369
TGACGGC3100.00770397168.90319946
Produced by FastQC (version 0.11.5)