FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072103

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072103
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5993926
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTATA9050.049.2103731
CGGCGTA1150.045.0307271
GGCCCCC49400.045.006421
CGGTACA7650.044.6775441
GGCCCTT65200.043.472151
GCGCATT9400.043.338081
GCGCCTT15300.043.0980341
GCGCCTA7450.043.096521
GCGTATA7850.042.6596831
GCGGCTA6800.042.1390461
CGGTATT13150.041.743311
GCGACTT8450.041.6734051
GGCCCAT64250.041.64321
CGGTATG8300.041.5946461
CGGTCCT9900.041.498011
GCGACTC5350.041.299211
GGCCCTC60750.041.257781
GCGTCTC12050.041.2563671
GCGACTA5700.041.1859971
GCGCCCT12200.041.0320971
Produced by FastQC (version 0.11.5)