FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072105

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072105
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5120212
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCTA7150.048.7685281
GCGACCA6400.048.5497321
CGCGTAT800.047.437012
CGGTATA7250.047.1434631
GCGACTT6750.047.0552831
GCGCCTT11500.046.2324831
GCGACTA3300.046.0323371
GCGCGTA900.046.0323371
GCGACCC5150.044.915051
CGGACCA4700.044.8080751
GGCCCAT53050.044.4487571
GCGCCTC11600.044.0481871
GGCCCTT52400.043.8800661
CGGTATC5550.043.5441061
GCGTCTT8050.043.3161431
GCGACTG6150.043.2254871
GCGCCCT9350.043.2014731
CGGTACA5600.043.155321
CCGGATA2100.042.7443161
GGCGGAC2350.042.60441
Produced by FastQC (version 0.11.5)