FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072106

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072106
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5120212
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA69340.1354240800966835No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA2207.2759576E-1221.95593369
TCGCCGT2551.36788E-917.58934668
GCGTCGG10350.016.66771969
TAGCCGG12850.015.5719022
TCTCGGG3907.2759576E-1215.03941269
GTGGTCG10100.015.03065169
GGTCGCC6050.014.82738369
TTAGCCG12850.013.9623791
CGGGCAT7150.013.0279046
GTCGTGG7250.012.84908769
GTCGCCG3651.5420483E-712.28844669
GGCGCCT6900.011.99993720
TGGTCGC7800.011.94306368
ACCGTAG2601.3470827E-411.942245516
CTACTCG11000.011.91811937
TCGGTGG19000.011.44019569
GCCGTAT3054.7115394E-511.3121969
GGTGGCG14950.011.07686413
TGGCGGG9400.011.01058115
CGATATG2200.00631041610.9782881
Produced by FastQC (version 0.11.5)