FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072109

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072109
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3909300
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTACT2750.051.4649051
GCGACTA3050.047.534561
GCGTACA3950.047.190731
GCGCGTA1100.047.071561
GCGTATC3300.047.071561
GCGCCTA6450.046.0255241
CGGTCCA6050.045.645151
GCGTACC2500.045.5652731
GCGCCCT7350.045.086231
GCGCATA3600.045.066661
CGGTATT7400.044.781591
GGCCCAT36850.044.4955061
CGGTACT4900.044.3817561
CGGTATA6000.043.724251
GCGACTT5300.043.637411
GCGACCA4650.043.056141
GCGTATT5150.042.897581
GCGGGTT4750.042.876411
CGGTATG4750.042.876411
CGGTCCT4800.042.4297871
Produced by FastQC (version 0.11.5)