FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072110

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072110
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3909300
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA66080.16903281917478832No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG10400.026.20952269
CGCCGTA1507.3339106E-720.70220669
GTCGTGG5900.016.37462669
TCGCCGT2201.6211379E-615.6834968
TCTCGGG3151.3587851E-915.33496869
GTCGCCG3204.1825842E-712.93854767
GGTCGCC4852.3646862E-1112.80548969
AGAGCGG16550.011.46647869
TGGTCGC5804.3655746E-1111.30292869
CTACTCG7100.011.175961537
CGTGCAA2300.00877813910.5008511
GTGGTCG6352.3283064E-1010.323935568
TACTCGG7503.6379788E-1210.11991438
CGTCGGG6251.9299478E-99.93680567
CTCGGGA7901.2732926E-119.60751340
CGGGCGT4704.0085615E-69.5424726
ATCTCGG19750.09.43391769
GTAGTCC18450.09.34963327
TTAGCCG8505.456968E-129.3360511
TAGCCGG8659.094947E-129.1733312
Produced by FastQC (version 0.11.5)