FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072112

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072112
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4727859
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA78440.16591019317623473No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG7950.023.43698769
CGCCGTA2103.6379788E-1223.00296869
GTCGCCG3900.016.80986268
TCGCCGT2702.923116E-916.61325568
GGTCGCC5800.014.87260969
TGGTCGC7300.013.70724869
GTGCGTT5350.013.54194359
GTCGTGG5053.6379788E-1212.981873569
TCTCGGG3204.1807652E-712.9391769
TGCGTTC5700.012.7104260
TCGTATA4154.8567017E-912.46977511
TTCGTAT4308.230927E-912.03478110
GCGTTCA5853.6379788E-1211.79477261
CGGGATT8000.011.6442681
CGGTGGG6850.011.58543769
GTGGTCG9250.011.56365569
TCGGTGG17500.011.43576268
CTTCGTA4652.6175258E-811.1289399
TTAGCCG10300.011.0538891
CGGTGGT14900.010.88395568
Produced by FastQC (version 0.11.5)